KMID : 0380020130280010031
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Korean Journal of Biotechnology and Bioengineering 2013 Volume.28 No. 1 p.31 ~ p.35
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Study on Cell Growth Characteristics with Culture Medium Components by Using MABOOMSTM
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Sohn Ok-Jae
Rhee Jong-Il
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Abstract
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In this work a MABOOMSTM has been employed to cultivate microorganisms and investigated the effects of culture medium components on cell growth. A 24-well microplate coated with 4-divided fluorescent sensing membranes was used to monitor the dissolved oxygen, pH and cell concentration during cultivations. Fluorescence intensity for dissolved oxygen or solution pH and reflectance for cell concentration was online monitored by using the MABOOMSTM. The online monitoring results showed the effects of culture medium components on cell growth in cultivation processes very well.
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KEYWORD
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culture medium, fluorescent sensing membrane, MABOOMSTM, Online monitoring
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